Liberatori
Tokumaru
who were blinded to any patient information. Brait
Yamazaki
Objective: We sought to determine the efficacy of increased expression of protein gene product 9.5 (PGP9.5), encoded by ubiquitin carboxyl-terminal esterase L1 (UCHL1) as an additional marker to loss of parafibromin immunoreactivity for the diagnosis of parathyroid carcinoma. JE
USA.gov.
In these areas, cancer cells were mainly irregular or not gland-forming, ie, an invasive growth pattern at the invasive front.30 On the other hand, stroma with fine and elongated fibers and thin spindle-shaped fibroblasts that were stratified into multiple layers in and around cancer, ie, an expanded growth pattern,30 showed weak or sparse PGP9.5 expression. et al.
Overall (A) and recurrence-free (B) survival of patients in relation to PGP9.5 expression (P = .002 and P < .001, respectively). Due to the frequent lack of S100 protein expression in malignant peripheral nerve sheath tumors (MPNSTs), especially the epithelioid variant, these tumors are difficult to diagnose without the aid of electron microscopy or a clinical history of neurofibromatosis. Carvalho
MS
Growth-associated protein 43 in differentiating peripheral nerve sheath tumors from other non-neural spindle cell neoplasms. Y
14, 15 As for the relation of PGP9.5 to cancer cells, its up-regulation is demonstrated in various tumors, including leukemia; some types of carcinomas such as esophageal, colorectal, pancreatic, lung, and breast; and various mesenchymal neoplasms such as nerve sheath, … When there is a lack of S100 protein expression and a broad panel of immunostains, such as cytokeratin, epithelial membrane antigen, and smooth muscle actin, yields only focal or equivoc …
B, α-SMA expression is absent in untreated fibroblasts (×500).
| Larsen
Ino
. CAFs obtained from colorectal cancer tissues were lysed on ice for 30 minutes with lysis buffer (RIPA buffer, 1 mmol/L calcium chloride, 1 mmol/L magnesium chloride, and protease inhibitor cocktail tablets; Roche Diagnostics, Mannheim, Germany). Samples of 10 μg of protein per lane were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to an Immobilon-P transfer membrane (Millipore, Billerica, MA) as described previously.20 After blocking, the membranes were incubated with NCC-2711 antibody followed by incubation with peroxidase-conjugated secondary antibody (GE Healthcare, Little Chalfont, England).
Copyright © 2020 American Society for Clinical Pathology. It has been so far reported that there are multiple processes involved in the desmoplastic reaction that exert opposing effects on cancer behavior.32 These different processes may independently control neoplastic growth or can occur simultaneously at different locations in the same tumor.
Conclusion:
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C
The tissue was minced into 2- to 3-mm fragments and seeded into Dulbecco modified Eagle medium (Sigma, Tokyo, Japan) containing 10% fetal bovine serum and penicillin/streptomycin (Invitrogen, Carlsbad, CA).11 Specifically, the procedure, which is based on the selective growth advantage of fibroblasts under the culture conditions used, allowed for a 100% pure fibroblast population, as confirmed by positive staining for vimentin and α-SMA and negative staining for cytokeratin (data not shown). PGP9.5 is a member of the ubiquitin C-terminal hydroxylase family and is … Seshimo
Dembinski
III
M
ML
On the other hand, stroma with fine and elongated collagen fibers and thin spindle-shaped fibroblasts that were stratified into multiple layers in and around cancer showed weak or sparse PGP9.5 expression Image 2E. Mareel
2006 May;19(5):659-68. doi: 10.1038/modpathol.3800569.
Multivariate analysis indicated PGP9.5 expression as an independent prognostic factor for overall and recurrence-free survival partly as well as lymph node metastasis.
After deparaffinization, all sections were subjected to antigen retrieval in citrate buffer (10 mmol/L, pH 6.0) at 121°C for 10 minutes. T
The relationships between clinicopathologic characteristics and the proportion of PGP9.5-immunopositive fibroblasts were analyzed by variance test as appropriate. DC
AB
Chiu
The mechanism whereby PGP9.5 expression in fibroblasts works as an independent prognostic factor is still unclear, but its expression practically predicts the tumor aggressiveness.
Krantz
Postsurgical follow-up studies were completed for all patients, with follow-up periods ranging from 126 to 2,452 days (median, 1,999 days). Some of these established antibodies were immunopositive with smooth muscle cells and CAFs or with fibroblasts weakly.
As controls, 6 monophasic synovial sarcomas, 9 leiomyosarcomas, and 5 dermatofibrosarcoma protuberans were included.
Expression of PGP9.5 was seen in 15 MPNSTs, with 3 to 4+ positivity in the majority of the cases.
Yamazaki
Cellular neurothekeoma (CNTK) frequently enters the differential diagnosis of a benign dermal cellular proliferation. Part II: Malignant neoplasms.
Nakazawa
et al. S
Xu
Yamashita
Independently reviewed in 5 review(s).
Some mAbs or polyclonal antibodies against PGP9.5 are commercially available.
CN
Nagasaka
M
A, PGP9.5 shows a punctate staining pattern in the nucleus and weak staining in the cytoplasm of untreated fibroblasts (×500).
This study was approved by the Ethics Committee of the National Cancer Center, Tokyo. Cited in 10 publication(s). The present study demonstrates that PGP9.5 expressed in CAFs can be considered a new useful marker for the biologic behavior of colorectal cancer. Sobin
WJ
S
The tumors were categorized by the ratio and localization of immunopositive fibroblasts in the sections of maximum tumor diameter. et al.
Bissell
Jass
Samples were examined with a confocal microscope (LSM5 PASCAL; Carl Zeiss Jena, Jena, Germany) equipped with a 15-mW krypton/argon laser. P
T
PGP9.5 expression in cultured normal dermal fibroblasts was induced by addition of TGF-β, simultaneously exhibiting α-SMA expression. Subsequently, the cells were fixed and stained. The results of univariate analysis using the Cox proportional hazards model are shown in Table 2. Wilkinson
To assess the expression of a cancer-associated fibroblasts (CAFs) marker as an indicator of prognosis, we raised anti–protein gene product 9.5 (PGP9.5) monoclonal antibody against cultured fibroblasts. Postsurgical recurrence was diagnosed by ultrasonography and computed tomography.
As an internal positive control for PGP9.5 staining, the immunopositivity of normal neural cells and nerve fibers in neuroplexus was used. In this study, we assessed PGP9.5 expression in CAFs in colorectal cancer by immunohistochemical analysis using the antibody we raised, and explored the alterations in PGP9.5 expression in cultured dermal fibroblasts with transforming growth factor (TGF)-β stimulation. PGP9.5 expression in cultured normal fibroblasts was increased by transforming growth factor β stimulation, indicating the phenotypic alteration to activated fibroblast. Hibi
Y
K
Normal human dermal fibroblasts were grown on coverslips and treated with TGF-β for 3 days. Of the 110 cases, 55 (50.0%) were classified as stage II and 55 (50.0%) as stage III. Each fibroblast fraction was used to immunize a BALB/c mouse, and hybridomas were produced as described previously.20 The obtained monoclonal antibodies (mAbs) were selected on the basis of fibroblast staining of formalin-fixed, paraffin-embedded sections of several cancer tissues.
M
Lamps
Boraldi
We have confirmed that rabbit anti-PGP9.5polyclonalantibody(dilution1:100,DakoCytomation, Carpinteria, CA) had the same immunoreactivity as our established anti-PGP9.5 mAb in cancer sections (data not shown). © 2016 Royal College of Pathologists of Australasia. Protein gene product 9.5 (PGP9.5), known as ubiquitin carboxyl-terminal hydrolase L1, that catalyzes the hydrolysis of C-terminal ubiquitin esters and amides has an important role in protein degradation through the recycling of free ubiquitin by cleaving ubiquitinylated peptides.13 PGP9.5 expression is found in fibroblasts in vivo and in vitro.
G, Western blotting of fibroblasts untreated or treated with TGF-β and neutralized TGF-β by anti–TGF-β monoclonal antibody. PGP9.5 expression in the cancer stroma was also associated with TNM stage (P = .019) and tended to also associate with lymph node metastasis (P = .054).
Although PGP9.5 is not a specific marker for MPNST, it is a more sensitive marker than S100 protein (94% vs 38%).
Demetter
Li
K
Although PGP9.5 is not a specific marker for MPNST, it is a more sensitive marker than S100 protein (94% vs 38%). H
Epub 2017 Mar 14. TGF-β (2 ng/mL; R&D Systems, Minneapolis, MN) was added, and, after 3 days, the cells were subjected to immunofluorescence staining and Western blotting. As an internal positive control for PGP9.5 staining, normal neural cells and nerve fibers were strongly positive for PGP9.5, whereas PGP9.5 expression was absent in smooth muscle cells and endothelia Image 2B. Immunohistochemical studies of PGP9.5 in advanced colorectal carcinoma. PGP9.5 expression in the cancer stroma was found to be a significant prognostic factor for overall and recurrence-free survival together with lymph node metastasis. Patients with tumors exhibiting high PGP9.5 expression showed significantly shorter survival than patients with tumors exhibiting low PGP9.5 expression (P = .002 and P < .001, respectively; log-rank test for overall and recurrence-free survival rates) Figure 1. Volume 168, Issue 1. Immunocytochemical studies with PGP9.5 (red) and α-SMA (green) staining are shown. Kim
Ten cases, 2 epithelioid and 8 conventional MPNSTs, were reactive with PGP9.5, but were negative for S100 protein. DS
Tezel
Moreover, by multivariate analysis, the percentage of cells expressing PGP9.5 in the stroma was an independent factor predictive of overall and recurrence-free survival.
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